Posted on Augus 8th, 2015 at 6:00 PM updated: 10/03/2015 10:21 AM

Tags: Curriculum Vitae

 

 

Walter Gabriel Gonzalez

wgonz002@gmail.com / www.chembiophys.com

 

Education

Doctor of philosophy in Chemistry                                                                                                 2011-Present

Florida International University - Miami, Florida USA                                                                    

 Ph. D. thesis on the structural mechanism of signal transduction across the voltage-gated K+ channel by the calcium sensing protein KChIP3, under supervision of Dr. Jaroslava Miksovska.

Bachelor degree in Physics                                                                                                               2006-2010

Florida International University - Miami, Florida USA

 

Professional experience

PhD Research Fellow                                                                                                                       2011-present

Department of Chemistry and Biochemistry, Florida International University, Miami, Florida USA

  • Optimized recombinant protein expression and characterization using a wide array of spectroscopic and calorimetric techniques.
  • Designed, developed, and analyzed experiments to elucidate molecular mechanism of ligand-protein interactions as well as all aspects of scientific manuscript preparation and submission.
  • Assembled, optimized, and serviced spectroscopic and photothermal equipment as well as computer clusters.
  • Supervised and trained new laboratory members.

Laboratory Technician                                                                                                                     2010-2011 Cell Science Systems, Deerfield Beach, Florida USA

  • In charge of antigen purification from a varied array of natural sources under strict SOP and clean room ISO 14644 class 3 standards.
  • Directly reported to lab manager on instrument maintenance, inventory and work progress.
  • Monitored and conducted quality control on production process.
  • Received largest departmental salary raise and outstanding employee recognition award.

 

Peer Reviewed Publications

  • Gonzalez Walter G; Perez, David; Mayorga, Norman; and Miksovska, Jaroslava; Structure-based analysis of ligand binding to DREAM. Manuscript in preparation (expected submission April 2016)

 

  • Gonzalez Walter G; David, Perez; Diaz, Maurizio; Vila, Ana; and Miksovska, Jaroslava; Allosteric network in the neuronal calcium sensor DREAM. Manuscript in preparation (expected submission March 2016)

 

  • Gonzalez Walter G; Ramos, Victoria; Diaz, Maurizio; and  Miksovska, Jaroslava; Photophysics, thermodynamics and structural properties of the Terbium(III)-KChIP3 complex. Manuscript in preparation (expected submission November 2015)

 

  • Gonzalez, Walter G; Andres S. Arango; Miksovska, Jaroslava; (2015) Amphiphilic residues 29-44 of DREAM N-termini mediate the calmodulin:DREAM complex formation. Biochemistry; 54 (28), 4391-4403.

 

  • Jessica Zayas, Marie Annoual, Jayanta Kumar Das, Quentin Felty, Walter G. Gonzalez, Jaroslava Miksovska, and Stanislaw F. Wnuk; (2015) Strain Promoted Click Chemistry of 2- or 8-Azidopurine and 5-Azidopyrimidine Nucleosides and Nucleotides with Cyclooctynes. Bioconjugate Chemistry; 26 (8), 1519-1532.

 

  • Haiyang Liu, Richard Lantz, Patrick Cosme, Nelson Rivera, Carlos Andino, Walter G. Gonzalez, Andrew C. Terentis, Ewa P. Wojcikiewicz, Rolando Oyola, Jaroslava Miksovska and Deguo Du; (2015) Site-Specific Dynamics of Amyloid Formation and Fibrillar Configuration of Aβ1-23 Using an Unnatural Amino Acid. Chemistry Communications; 51(32): 7000-3.

 

  • Khoa Pham, Gangadhar Dhulipala, Walter G. Gonzalez, Bernard S. Gerstman. Chola Regmi, Prem P. Chapagain, and Jaroslava Miksovska; (2015)Ca2+ and Mg2+ modulate conformational dynamics and stability of Downstream Regulatory Element Antagonist Modulator. Protein Science; 24(5): 741-51.

 

  • Gonzalez, Walter G; Pham, Khoa; Miksovska, Jaroslava; (2014) Modulation of the Voltage-gated Potassium Channel (Kv4. 3) and the Auxiliary Protein (KChIP3) Interactions by the Current Activator NS5806. Journal of Biological Chemistry; 289 (46): 32201-32213.

 

  • Molano-Arevalo, Juan Camilo; Hernandez, Diana R.; Gonzalez, Walter G; Miksovska, Jaroslava; Ridgeway, Mark E.; Park, Melvin A.; Fernandez-Lima, Francisco; (2014) Flavin Adenine Dinucleotide structural motifs: from solution to gas-phase. Analytical Chemistry, 86 (20): 10223–10230

 

  • Gonzalez, Walter G; Miksovska, Jaroslava; (2014) Application of ANS fluorescent probes to identify hydrophobic sites on the surface of DREAM. Biochimica et Biophysica Acta-Proteins and Proteomics; 1844 (9): 1472-1480.

 

  • Gonzalez, Walter G; Miksovska, Jaroslava; (2014) Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection. JoVE (Journal of Visualized Experiments); 8; e50969-e50969.

 

  • Dhuguru, Jyothi; Liu, Wenjun; Gonzalez, Walter G; Babinchak, William Michael; Miksovska, Jaroslava; Landgraf, Ralf; Wilson, James N; (2014) Emission Tuning of Fluorescent Kinase Inhibitors: Conjugation Length and Substituent Effects. Journal of organic Chemistry; 79 (11): 4940–4947.

 

  • Li, Shanghao; Aphale, Ashish N; Macwan, Isaac G; Patra, Prabir K; Gonzalez, Walter G; Miksovska, Jaroslava; Leblanc, Roger M; (2012) Graphene Oxide as a Quencher for Fluorescent Assay of Amino Acids, Peptides, and Proteins. ACS applied materials & interfaces;        4 (12): 7069-7075.

 

  • Adjaye-Mensah, Edward; Gonzalez, Walter G; Bussé, David R; Captain, Burjor; Miksovska, Jaroslava; Wilson, James N; (2012) Emission Switching of 4, 6-Diphenylpyrimidones: Solvent and Solid State Effects. The Journal of Physical Chemistry A; 116 (34): 8671-8677.

 

  • Adjaye-Mensah, Edward; Gonzalez, Walter G; Miksovska, Jaroslava; Wilson, James N; (2012) Photophysical Characterization of a Benzo-Fused Analogue of Brooker’s Merocyanine: Solvent Polarity and pH Effects     . The Journal of Physical Chemistry A; 116 (51): 12470-12475.

 

  • Gantar, M; Simovic, D; Djilas, S; Gonzalez, Walter, G; Miksovska, J; (2012) Isolation, characterization and antioxidative activity of C-phycocyanin from Limnothrix sp strain 37-2-1. Journal of Biotechnology; 159 (1-2): 21-26.

 

 

 

Awards

  • Graduate Presentation 1st place, 2015 MARC U*STAR & MBRS RISE Mini-Symposium            2015
  • Summer Research Award, NIH Biomedical Research Initiative.                                                      2015

Role of DREAM protein dynamics and oligomerization on ligand association.

  • Graduate Presentation 2nd prize, 13h Raymond N. Castle Student Research Conference.                2015
  • MBRS RISE Graduate Fellowship, Florida International University.                                    2015-present
  • Biophysical Society Travel Award, Biophysical Society.                                                                  2015
  • Summer Research Award, NIH Biomedical Research Initiative.                                                      2014

Mechanism of Ca2+ and NS5806 induced KChIP3-Kv4.3 association.

  • Graduate Poster Presentation 2nd prize, 11h Raymond N. Castle Student Research Conference.      2013
  • Summer Research Award, NIH Biomedical Research Initiative.                                                      2013

Structural modulation of Kv4.3 T1 domain by the calcium binding protein KChIP3.

  • ACS South Florida travel award, American Chemical Society.                                                   2013
  • Graduate Research Award, FIU Dept. of Chemistry and Biochemistry.                                          2012
  • Graduate Poster Presentation 2nd prize, Florida Annual Meeting and Exposition.                            2011

 

Training Workshops

Principles of Fluorescence Technique Course, Champaign, Illinois.                          April 7-11th, 2014

12th Advanced Imaging methods Workshop, UC Berkeley, California.                                February 4-6th, 2015

10th LFD Workshop in Advanced Imaging, UC Irvine, California.                                     October 26-30th, 2015

 

Presentations

  • 2015 MARC U*STAR & MBRS RISE Biomedical Research Mini-Symposium                             2015
  • 250th ACS National Meeting and Exposition; Boston, Massachusetts.                                       2015
  • 91st Florida Annual Meeting and Exposition of the ACS; Tampa, Florida.                                       2015
  • 13h Raymond N. Castle Student Research Conference; Tampa, Florida.                                          2015
  • 17th Comparative Immunology Symposium; Miami, Florida. (Poster)                                        2015
  • Biophysical Society 59th Annual Meeting; Baltimore, Maryland. (Poster)                                        2015
  • South East Regional Meeting of the ACS; Nashville, Tennessee.                                                     2015
  • 16th Comparative Immunology Symposium. Miami, Florida.                                                     2015
  • Biophysical Society 58th Annual Meeting; San Francisco, California. (Poster)                           2014
  • Biophysical Society 57th Annual Meeting; Philadelphia, Pennsylvania.(Poster)                          2013
  • 245th ACS National Meeting and Exposition; New Orleans, Louisiana. (Poster)                              2013
  • South East Regional Meeting of the ACS; Raleigh, North Carolina.                                                2012
  • 89th Florida Annual Meeting and Exposition of the ACS; Tampa, Florida. (Poster)                         2012
  • 11th Raymond N. Castle Student Research Conference; Tampa, Florida. (Poster)                            2012
  • Biophysical Society 56th Annual Meeting; San Diego, California. (Poster)                                       2012
  • South East Regional Meeting of the ACS; Richmond, Virginia.                                                 2012
  • 88th Florida Annual Meeting and Exposition of the ACS; Tampa, Florida. (Poster)                         2011   
  • FIU Graduate symposium; Miami, Florida. (Poster)                                                                          2011

 

Invited Talks

  • Gonzalez, G. Walter. (2015) Protein interactions and allosteric control in calcium binding proteins. Undergraduate Senior Seminar,  Florida International University.

 

Conference Abstracts

 

  • Gonzalez, Walter G; Miksovska, Jaroslava; (2015) Characterization of the photophysical, thermodynamic and structural properties of the Terbium(III)-KChIP3 complex. Biophysical Journal; 108 (2), pp 218a.

 

  • Gonzalez, Walter G; Arango, Andres; Miksovska, Jaroslava; (2015) The N- terminal extension of KChIP-3 is responsible for KChIP-3: calmodulin complex formation. Biophysical Journal; 108 (2), pp 279a.

 

  • Gonzalez, Walter G; Andres S. Arango; Miksovska, Jaroslava; (2015) Interaction-​based analysis of the mechanism by which the extended amphiphilic N-​terminus of DREAM mediates calmodulin binding. Abstracts, 66th Southeast Regional Meeting of the American Chemical Society, Nashville, TN, United States, October 16-19, PagesSERMACS-566.

 

  • Gonzalez, Walter G; Miksovska, Jaroslava; (2014) Study of the Dynamic and Thermodynamic Calcium Induced Transition in the Downstream Regulatory Element Antagonist Modulator (DREAM) using Photothermal Beam Deflection (PBD). Biophysical Journal; 106  (2), pp 659a.

 

  • Gonzalez, Walter; Miksovska, Jaroslava; (2013) Modulation of the KV4. 3-KchIP3 Interactions by Ca2+ and NS5806. Biophysical Journal; 104 (102)

 

  • Gonzalez, Walter; Miksovska, Jaroslava; (2012) Ca+2 and Mg2+ Induced Conformational Changes in Downstream Regulatory Antagonist Modulator (DREAM) Measured by Extrinsic Hydrophobic Probes. Biophysical Journal 102 (3), pp 507a.

 

  • Gonzalez, Walter; Miksovska, Jaroslava; (2011) Ca2+ and Mg2+ induced conformational changes on DREAM (Downstream Regulatory Element Antagonist Modulator) measured by extrinsic hydrophobic probes. Abstracts, 63rd Southeast Regional Meeting of the American Chemical Society, Richmond, VA, United States, October 26-29, PagesSERM-484.

 Memberships

  • American Heart Association                                                                                             2013 – present
  • American Chemical Society                                                                                              2011 – present
  • Biophysical Society                                                                                                          2011 – present

 

Courses Taught

Florida International University

Summer 2014

CHM1046L – General chemistry laboratory II (two sections  enrollment: 30)

Fall 2014

CHM1046L – General chemistry laboratory II (two sections  enrollment: 35)

Spring 2015

CHM1046L – General chemistry laboratory II (two sections  enrollment: 27)

 

Teaching/Advising/Supervising students

Graduate

  • Samiol Azam                     (08/2015 to present)

 

Undergraduates

  • Joseph A. Hernandez        (06/2015 09/2015)
  • Dorian Delgado                (06/2015 to 09/2015)
  • Andres Arango                 (06/2012 to present) - 2nd place poster award.
  • Ana Vila                            (12/2014 to 08/2015)
  • Victoria Ramos                 (09/2014 to present)
  • David Perez                      (04/2015 to 07/21/2015)
  • Antonio Santana              (12/2015 to present)
  • Norman Mayorga              (05/2013 to 04/2014)

 

High School

  • Maurizio Diaz (04/2014 to present) – 2nd place presentation award.
  • Anna Rondon (04/2013 to 08/2013)

 

References

Dr. Jaroslava Miksovska

Associate Professor

Florida International University

Dr. Randy Larsen

Professor/Dept. Chair

University of South Florida

Dr. James Wilson

Associate Professor

University of Miami